Journal: Acta Pharmaceutica Sinica. B

Article Title: Remodeling of the liver fibrosis microenvironment based on nilotinib-loaded multicatalytic nanozymes with boosted antifibrogenic activity

doi: 10.1016/j.apsb.2023.08.020

Figure Lengend Snippet: In vitro antioxidative and antifibrogenic activities of APNH NTs to inhibit HSCs activation. (A) Schematic depiction of the antifibrogenic mechanism of APNH NTs scavenging NOX-4-generated ROS. (B) Flow cytometry analysis of ROS scavenging in LX-2 cells after different treatments for 24 h analyzed by DCFH-DA staining. (C) CLSM micrographs of total intracellular ROS, H 2 O 2 and O 2 .- in LX-2 cells exposed to the noted treatments for 24 h. Scale bar, 20 μm. (D) Quantitation of total ROS levels in LX-2 cells. Semiquantitative evaluation of CLSM micrographs showing (E) total ROS, (F) H 2 O 2 and (G) O 2 .- . G1: control, G2: model (LPS), G3: LPS + Ag@Pt NTs (50 μg/mL), G4: LPS + NIL (50 μg/mL), G5: LPS + APNH NTs (50 μg/mL), and G6: LPS + APNH NTs (100 μg/mL). Data are shown as the mean ± SD ( n = 3). NS (no significance), ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: DMSO, 4′,6-diamidino-2-phenylindole (DAPI), Hoechst 33342, LysoTracker Red, Calcein/PI cell viability assay kit, PBS, lipopolysaccharide (LPS), ROS probe (2,7-dichlorodihydrofluorescein diacetate, DCFH-DA), hydrogen peroxide probe (ROS GreenTM H 2 O 2 ), superoxide anion probe (dihydrorethidium, DHE), Annexin V-FITC apoptosis detection kits, as well as SOD and CAT activity assay kits were all purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China).

Techniques: In Vitro, Activation Assay, Generated, Flow Cytometry, Staining, Quantitation Assay, Control

Journal: Acta Pharmaceutica Sinica. B

Article Title: Remodeling of the liver fibrosis microenvironment based on nilotinib-loaded multicatalytic nanozymes with boosted antifibrogenic activity

doi: 10.1016/j.apsb.2023.08.020

Figure Lengend Snippet: In vitro activity of APNH NTs to alleviate hypoxia and deplete collagen. (A) Schematic depiction of the antifibrogenic activity of APNH NTs through hypoxia alleviation and collagen depletion. (B) The expression of HIF-1 α in LX-2 cells cultured in a hypoxic environment and exposed to the noted treatments for 24 h. G1: control (normoxia), G2: model (hypoxia), G3: hypoxia + Ag@Pt NTs (50 μg/mL), G4: hypoxia + NIL (50 μg/mL), G5: hypoxia + APNH NTs (50 μg/mL), and G6: hypoxia + APNH NTs (100 μg/mL). (C) The expression of NOX-4, TIMP-1, collagen-I and α -SMA in LX-2 cells exposed to the noted treatments for 24 h. G1: control (normal), G2: model (LPS), G3: LPS + Ag@Pt NTs (50 μg/mL), G4: LPS + NIL (50 μg/mL), G5: LPS + APNH NTs (50 μg/mL), and G6: LPS + APNH NTs (100 μg/mL). (D) CLSM micrographs of the intracellular hypoxia levels in LX-2 cells cultured under normoxic or hypoxic conditions and treated with the noted treatments for 24 h. Scale bar, 25 μm. (E) The expression of α -SMA and collagen-I examined by immunofluorescence after different treatments for 24 h. Scale bar, 50 μm. Data are shown as the mean ± SD ( n = 3). NS (no significance), ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: DMSO, 4′,6-diamidino-2-phenylindole (DAPI), Hoechst 33342, LysoTracker Red, Calcein/PI cell viability assay kit, PBS, lipopolysaccharide (LPS), ROS probe (2,7-dichlorodihydrofluorescein diacetate, DCFH-DA), hydrogen peroxide probe (ROS GreenTM H 2 O 2 ), superoxide anion probe (dihydrorethidium, DHE), Annexin V-FITC apoptosis detection kits, as well as SOD and CAT activity assay kits were all purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China).

Techniques: In Vitro, Activity Assay, Expressing, Cell Culture, Control, Immunofluorescence